A molecularly defined and spatially resolved cell atlas of the whole mouse brain

Abstract

In this project, we used MERFISH to perform in situ gene expression profiling of individual cells in the entire mouse brain, determine the cell-type identity of these cells and map the spatial organization of the cell types. We performed MERFISH imaging on 1124 or 1147 genes in a total of 245 brain slices (217 coronal, 28 sagittal) from a total of 4 animals.

Contributors

• Jung, Won ORCID Harvard University Researcher
• Zhang, Meng ORCID Harvard University Researcher
• Zhuang, Xiaowei ORCID Harvard University ProjectLeader
• Zhuang, Xiaowei ORCID Harvard University ContactPerson

Funding

• National Institutes of Health 5-U19-MH114830-05 A comprehensive whole-brain atlas of transcriptomic cell types in the mouse

Data