Mesoscopic analysis of GABAergic marker distribution in cholinergic neurons in the mouse brain: Raw data

[ BIL ID: ace-owl-cow ]

Dataset Citation:

Goral, Rene Oliver, Roy, Snehashis, Goral, Rene Oliver. (2025). Mesoscopic analysis of GABAergic marker distribution in cholinergic neurons in the mouse brain: Raw data [Dataset/Microscopy]. Brain Image Library.

 

Abstract:

This project explores the distribution of acetylcholine (ACh) neurons which co-express GABAergic markers in the mouse brain. GABAergic marker co-expression may explain which cholinergic neuron populations have the potential to co-transmit the neurotransmitter GABA at cholinergic synapses. ACh/GABA co-transmission has been previously reported as contributor to neural circuit function in health and disease. However, the role and location of GABA co-transmission from ACh neurons remains unclear. Using transgenic animal models in combination with tissue clearing and light-sheet microscopy, we investigated which ACh neurons are GABA co-positive. With this approach, we assessed the role of GABA co-transmission in ACh neuron function and quantified ACh and ACh/GABA neuron sub-populations in the brain. Our results suggest that GABA co-transmission from ACh neurons is dispensable to maintain a physiologic ACh neuron count in the brain. Furthermore, we report that a large subset of ACh neurons were or can synthesize GABA and, therefore, have a partial GABAergic fate. However, most of these ACh neurons lack another GABAergic marker which would enable these neurons to release GABA. Taken together, we conclude that GABA co-transmission likely occurs only from a small population of ACh neurons restricted to few brain nuclei.

 

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Technical Information:

population imaging (Modality) / other (Technique)

 

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