Fluorescent microscopy of coronal sections from A2a-FlpO x RCE:FRT mouse: d1CreC2
[ BIL ID: ace-oat-toy ]
Dataset Citation:
Albarran, Eddy, Fushiki, Akira, Nelson, Anders, Chaimowitz, Corryn, Nikoobakht, Laudan, Peterka, Darcy, Costa, Rui M.. (2025). Fluorescent microscopy of coronal sections from A2a-FlpO x RCE:FRT mouse: d1CreC2 [Dataset/Microscopy]. Brain Image Library.
Abstract:
This dataset is part of a whole-brain imaging experiment describing the expression of Cre or FlpO recombinase in various regions of the brain from various newly-generated knock-in lines (D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO). Knock-in recombinse lines were crosses to fluorescent reporter lines (e.g., Ai9) and then animals were perfused and imaged using fluorescent microscopy. Coronal and/or sagittal sections are present for each line to visualize brain-wide expression of the recombinase activity.
Contributors and Roles:
Albarran, Eddy ( DataCollector ),
Columbia University
;
Fushiki, Akira ( DataCollector ),
Allen Institute
;
Nelson, Anders ( DataCollector ),
New York University
;
Chaimowitz, Corryn ( DataCollector ),
New York University
;
Nikoobakht, Laudan ( DataCollector ),
Columbia University
;
Peterka, Darcy ( ProjectLeader ),
Columbia University
;
Costa, Rui M. ( ProjectLeader ),
Allen Institute
Funding:
Aligning Science Across Parkinson's
( ASAP-020551 ) Distributed circuit dysfunction underlying motor and sleep deficits in a progressive mouse model of Parkinson’s disease
Technical Information:
population imaging (Modality)
/ other (Technique)
Instrument Metadata
| microscopetype | Upright |
| microscopemanufacturerandmodel | Nikon AZ100 Multizoom Slide Scanner |
| objectivename | |
| objectiveimmersion | Air |
| objectivena | |
| objectivemagnification | |
| detectortype | sCMOS |
| detectormodel | |
| illuminationtypes | |
| illuminationwavelength | |
| detectionwavelength | |
| sampletemperature |
Specimen Metadata
| localid | d1CreC2 |
| species | Mouse |
| ncbitaxonomy | NCBI:txid10090 |
| age | 60-70 |
| ageunit | Days |
| sex | Female |
| genotype | Strain_Name='D1-Cre';Cre_Driver_Line='Drd1';Reporter_Name='Ai9'; |
| organlocalid | d1CreC2 |
| organname | Brain |
| samplelocalid | d1CreC2 |
| atlas | |
| locations | |
| linkage | [] |
Image Metadata
| xaxis | left-to-right |
| obliquexdim1 | |
| obliquexdim2 | |
| obliquexdim3 | |
| yaxis | superior-to-inferior |
| obliqueydim1 | |
| obliqueydim2 | |
| obliqueydim3 | |
| zaxis | anterior-to-posterior |
| obliquezdim1 | |
| obliquezdim2 | |
| obliquezdim3 | |
| landmarkname | |
| landmarkx | |
| landmarky | |
| landmarkz | |
| number | 1,2,red,blue |
| displaycolor | (1.0, 0.0, 0.0),(0.0, 0.0, 1.0) |
| representation | |
| flurophore | |
| stepsizex | 0.9727637 |
| stepsizey | 0.9727637 |
| stepsizez | |
| stepsizet | |
| channels | |
| slices | |
| z | |
| xsize | |
| ysize | |
| zsize | |
| gbytes | |
| files | |
| dimensionorder |
License:
Data:
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/bb/c5/bbc51b9c65aa3ce7/a2aFlpoC1
Dataset download link:
https://download.brainimagelibrary.org/bb/c5/bbc51b9c65aa3ce7/a2aFlpoC1
Metadata download link:
https://api.brainimagelibrary.org/retrieve?bildid=ace-oat-toy