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Confocal microscope images a kinase inhibitor screen to identify kinases regulating neuronal vulnerability to synuclein inclusions

ace-nut-gel Creative Commons Attribution 4.0 International (CC BY 4.0)

Cite this dataset

Henderson, Michael X., Naman Vatsa, Breton, Libby. (2025). Confocal microscope images a kinase inhibitor screen to identify kinases regulating neuronal vulnerability to synuclein inclusions [Dataset/Microscopy]. Brain Image Library.

Abstract

The dataset comprises immunofluorescence images stained for MAP2, pS129-alpha-synuclein, NeuN, and DAPI. Neurons were induced to develop synuclein pathology by seeding with mouse alpha-synuclein preformed fibrils (mPFFs). Various kinase inhibitors were screened to identify kinases regulating neuronal vulnerability to synuclein inclusion formation. The inhibitors were tested at three different concentrations to evaluate their effect on mPFF-induced synuclein aggregation, as measured by the area covered by pS129-alpha-synuclein signal

Methods

A full protocol describing this work is available at dx.doi.org/10.17504/protocols.io.5jyl82ym8l2w/v1

Contributors

Funding

Technical Information
Modality
other
Technique
other

Instrument

microscopetype High content confocal
microscopemanufacturerandmodel Molecular Devices, ImageXpress Confocal HT.ai
objectivename Molecular devices, Apo Lambda
objectiveimmersion Air
objectivena 1.0
objectivemagnification 10X
detectortype sCMOS
detectormodel
illuminationtypes
illuminationwavelength 350-650nm
detectionwavelength 450-692nm
sampletemperature 25°C

Specimen

localid Set 1a
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1493
atlas
locations Hippocampus
linkage []
localid Set 1b
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1494
atlas
locations Hippocampus
linkage []
localid Set 2a
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1585
atlas
locations Hippocampus
linkage []
localid Set 2b
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 22
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1586
atlas
locations Hippocampus
linkage []
localid Set 3a
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1587
atlas
locations
linkage []
localid Set 3b
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1588
atlas
locations
linkage []
localid Set 4a
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 21
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1615
atlas
locations
linkage []
localid Set 4b
species Mouse
ncbitaxonomy NCBI:txid10090
age E18
ageunit DIV 22
sex Unknown
genotype Strain Name = 'CD1'
organlocalid
organname Brain
samplelocalid Plate 1616
atlas
locations
linkage []

Image

xaxis NA
obliquexdim1
obliquexdim2
obliquexdim3
yaxis NA
obliqueydim1
obliqueydim2
obliqueydim3
zaxis NA
obliquezdim1
obliquezdim2
obliquezdim3
landmarkname
landmarkx
landmarky
landmarkz
number 1,2,3,4,green,red,far red, DAPI
displaycolor (128, 128, 128)
representation MAP2, NeuN, pS129 synuclein, Nucleus
flurophore (Alexa-488, Alexa-594,Alexa-647, DAPI)
stepsizex 0.6083
stepsizey 0.6083
stepsizez
stepsizet
channels 4.0
slices 1.0
z 1.0
xsize
ysize
zsize
gbytes 66.3GB
files 17280.0
dimensionorder

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Data

Analysis Ecosystem path
/bil/data/b0/9e/b09e5989cb3f551a
Download
https://download.brainimagelibrary.org/b0/9e/b09e5989cb3f551a
Metadata
https://api.brainimagelibrary.org/retrieve?bildid=ace-nut-gel