Super-resolution retinogeniculate synapses during eye-specific segregation, WTP2A
[ BIL ID: ace-dud-lid ]
Dataset Citation:
Chenghang, Zhang. (2024). Super-resolution retinogeniculate synapses during eye-specific segregation, WTP2A [Dataset/Microscopy]. Brain Image Library.
Abstract:
Retinogeniculate synapses imaged using STORM microscope from P2 to P8. CTB-488 was injected one day prior to data collection. Main data is in the STORM_merged subfolders. In subfolder of each sample: STORM_merged: merged super-resolution images of VGluT2, Bassoon, and Homer123; Conv_merged: 3-color conventional images; Conv_488: conventional CTB-488 images. Storm_561_488: the blue channel is CTB-488 super-resolution.
Methods:
A full protocol describing this work is available at protocol https://doi.org/10.1016/j.xpro.2021.100971
Contributors and Roles:
Chenghang, Zhang ( DataCollector ),
University of Maryland, College Park
Funding:
National Institutes of Health
( 1-DP2-MH125812-01 ) A molecular connectomics platform for multi-scale analysis of activity-dependent synapse development and plasticity
Technical Information:
connectivity (Modality)
/ other (Technique)
Instrument Metadata
| microscopetype | STORM |
| microscopemanufacturerandmodel | Customized |
| objectivename | Zeiss |
| objectiveimmersion | Oil |
| objectivena | 1.4 |
| objectivemagnification | 60.0 |
| detectortype | CMOS |
| detectormodel | |
| illuminationtypes | Dye |
| illuminationwavelength | 488-750 nm |
| detectionwavelength | |
| sampletemperature |
Specimen Metadata
| localid | WTP2_A |
| species | Mouse |
| ncbitaxonomy | NCBI:txid10090 |
| age | 2.0 |
| ageunit | Days |
| sex | Unknown |
| genotype | Strain_Name='C57BL6'; |
| organlocalid | |
| organname | Brain |
| samplelocalid | WTP2_A |
| atlas | |
| locations | dLGN |
| linkage | [] |
Image Metadata
| xaxis | NA |
| obliquexdim1 | |
| obliquexdim2 | |
| obliquexdim3 | |
| yaxis | NA |
| obliqueydim1 | |
| obliqueydim2 | |
| obliqueydim3 | |
| zaxis | NA |
| obliquezdim1 | |
| obliquezdim2 | |
| obliquezdim3 | |
| landmarkname | |
| landmarkx | |
| landmarky | |
| landmarkz | |
| number | red, green, blue |
| displaycolor | (1,0,0),(0,1,0),(0,0,1) |
| representation | Protein |
| flurophore | (Bassoon, Homer123, VGluT2) |
| stepsizex | 15.5nm |
| stepsizey | 15.5nm |
| stepsizez | 70nm |
| stepsizet | |
| channels | 3.0 |
| slices | 72.0 |
| z | 1.0 |
| xsize | 4401.0 |
| ysize | 4421.0 |
| zsize | 72.0 |
| gbytes | 9.0 |
| files | 288.0 |
| dimensionorder |
Cited By:
License:
Data:
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/5a/1e/5a1e85918b52cd7c/WTP2_A
Dataset download link:
https://download.brainimagelibrary.org/5a/1e/5a1e85918b52cd7c/WTP2_A
Metadata download link:
https://api.brainimagelibrary.org/retrieve?bildid=ace-dud-lid
Manifest download link:
/bil/pscstaff/icaoberg/bil-inventory/datasets/json/ace-dud-lid.json