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Whole-brain LSFM imaging of a P14 mouse for DevCCF project (Subject: P14_BB0174)

ace-dub-ear Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)

Cite this dataset

Yongsoo Kim Lab, Kim, Yongsoo, Kronman, Fae, Liwang, Josephine, Minteer, Jennifer, Donghui, Shin. (2022). Whole-brain LSFM imaging of a P14 mouse for DevCCF project (Subject: P14_BB0174) [Dataset/Microscopy]. Brain Image Library.

Abstract

This dataset is part of a BICCN project to create developmental CCFswith associated ontology and true 3D anatomical labels while also demonstrating the application of our CCFsby generating quantitative mappings of GABAergic neurons in the developing mouse brain. This sample consist of LSFM imaging of a P14 mouse (Subject: P14_BB0174)

Methods

Images were processed using a modified LifeCanvas Protocol

Contributors

Funding

  • National Institutes of Health 1-RF1-MH124605-01 Establishing Common Coordinate Framework for Quantitative Cell Census in Developing Mouse Brains

Technical Information
Modality
population imaging
Technique
other
Details
Images were stitched and compressed using in house code with MATLAB and Python, respectively

Instrument

microscopetype Light sheet microscope (LSFM)
microscopemanufacturerandmodel Life Canvas Tech
objectivename
objectiveimmersion
objectivena
objectivemagnification
detectortype
detectormodel
illuminationtypes
illuminationwavelength
detectionwavelength
sampletemperature

Specimen

localid P14_BB0174
species Mouse
ncbitaxonomy NCBI:txid10090
age P14
ageunit Postnatal Days
sex Female
genotype Strain_Name='C57BL6'; Cre_Driver_Line=''; Reporter_Name='none'
organlocalid
organname Brain
samplelocalid P14_BB0174
atlas
locations
linkage []

Image

xaxis right-to-left
obliquexdim1
obliquexdim2
obliquexdim3
yaxis posterior-to-anterior
obliqueydim1
obliqueydim2
obliqueydim3
zaxis superior-to-inferior
obliquezdim1
obliquezdim2
obliquezdim3
landmarkname
landmarkx
landmarky
landmarkz
number 1,2,3
displaycolor 488 561 642
representation Background Autofluorescence, Lectin Fluorescent Stain, Neurotrace Fluorescent Stain
flurophore
stepsizex 1.873
stepsizey 1.873
stepsizez 5.0
stepsizet
channels
slices
z
xsize
ysize
zsize
gbytes
files
dimensionorder

Related Works

Cited by

Data

Analysis Ecosystem path
/bil/data/b4/d4/b4d41a4bf7614449/P14_BB0174/LSFM/
Download
BIL data will be unavailable between October 5 and October 16. Read More
https://download.brainimagelibrary.org/b4/d4/b4d41a4bf7614449/P14_BB0174/LSFM/
Metadata
https://api.brainimagelibrary.org/retrieve?bildid=ace-dub-ear