Level 1 whole-brain confocal imaging
[ BIL ID: ace-bug-wit ]
Dataset Citation:
Hong-Wei Dong. (2022). Level 1 whole-brain confocal imaging [Dataset/Microscopy]. Brain Image Library.
Abstract:
Viral tracers are used to sparsely label connectionally-unique projection neurons to assess their morphological characteristics. Following SHIELD-processing of 500-um thick sections, volumetric image stacks are acquired using a confocal microscope at 10x and 30x magnifications.
Contributors and Roles:
Center for Integrative Connectomics ( ResearchGroup )
;
Hong-Wei Dong ( ProjectLeader ),
University of California, Los Angeles
Funding:
National Institutes of Health
( 1-U01-MH114829-01 ) Anatomical Characterization of Neuronal Cell Types of the Mouse Brain
Technical Information:
connectivity (Modality)
/ retrograde transynaptic tracing (Technique)
Instrument Metadata
| microscopetype | Inverted Spinning Disk Confocal |
| microscopemanufacturerandmodel | Andor Dragonfly 200 |
| objectivename | Olympus 10x UPlanApo |
| objectiveimmersion | Air |
| objectivena | 0.4 |
| objectivemagnification | 10x |
| detectortype | |
| detectormodel | |
| illuminationtypes | |
| illuminationwavelength | 405, 488, 561, 637 |
| detectionwavelength | |
| sampletemperature | 27C |
Specimen Metadata
| localid | SW220310-01 |
| species | mouse |
| ncbitaxonomy | NCBI:txid10090 |
| age | 3.0 |
| ageunit | Months |
| sex | Male |
| genotype | C57BL6 |
| organlocalid | |
| organname | Brain |
| samplelocalid | SW220310-01 |
| atlas | AllenMousev1 |
| locations |
Image Metadata
| xaxis | left-to-right |
| obliquexdim1 | |
| obliquexdim2 | |
| obliquexdim3 | |
| yaxis | superior-to-inferior |
| obliqueydim1 | |
| obliqueydim2 | |
| obliqueydim3 | |
| zaxis | anterior-to-posterior |
| obliquezdim1 | |
| obliquezdim2 | |
| obliquezdim3 | |
| landmarkname | |
| landmarkx | |
| landmarky | |
| landmarkz | |
| number | 1,2,3,4,blue,green,red,far red |
| displaycolor | (Red, green, blue) |
| representation | |
| flurophore | |
| stepsizex | 0.603 |
| stepsizey | 0.603 |
| stepsizez | 2.0 |
| stepsizet | |
| channels | 4.0 |
| slices | |
| z | |
| xsize | |
| ysize | |
| zsize | |
| gbytes | |
| files | |
| dimensionorder |
License:
Data:
Neuroglancer Vizualization Links:
Data location on the Brain Image Library Analysis Ecosystem:
/bil/data/04/6c/046c91d35a983d7a/10x
Dataset download link:
https://download.brainimagelibrary.org/04/6c/046c91d35a983d7a/10x
Metadata download link:
https://api.brainimagelibrary.org/retrieve?bildid=ace-bug-wit
Manifest download link:
https://download.brainimagelibrary.org/inventory/59f647b7-29ab-5ec0-aabd-097250afa3ba.json